Establishment of a polymerase chain reaction method for detection of Escherichia coli in amniotic fluid in patients with chorioamnionitis.

@article{Daoud2008EstablishmentOA,
  title={Establishment of a polymerase chain reaction method for detection of Escherichia coli in amniotic fluid in patients with chorioamnionitis.},
  author={Ghada Abdel-Hamid Daoud and Yoshikatsu Suzuki and Tamao Yamamoto and Takashi Suzuki and Nobuhiro Suzumori and Masahiro Tanemura},
  journal={Fetal diagnosis and therapy},
  year={2008},
  volume={24 2},
  pages={
          132-9
        }
}
OBJECTIVE We investigated whether Escherichia coli could be detected by E. coli of reference (ECOR) grouping and virulence factors (VFs) in amniotic fluid using polymerase chain reaction (PCR). METHOD From 18 patients with clinical symptoms, such as cyclic uterine contraction, genital bleeding and cervical ripening, who subsequently developed abortion or labor before term, and from 40 normal pregnant women undergoing diagnostic amniocentesis, amniotic fluid was obtained. All samples were… CONTINUE READING
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