Establishment of a novel gene expression method, BICES (biomass-inducible chromosome-based expression system), and its application to the production of 2,3-butanediol and acetoin.

@article{Nakashima2014EstablishmentOA,
  title={Establishment of a novel gene expression method, BICES (biomass-inducible chromosome-based expression system), and its application to the production of 2,3-butanediol and acetoin.},
  author={Nobutaka Nakashima and Hironaga Akita and Tamotsu Hoshino},
  journal={Metabolic engineering},
  year={2014},
  volume={25},
  pages={
          204-14
        }
}
In this study, we describe a novel method for producing valuable chemicals from glucose and xylose in Escherichia coli. The notable features in our method are avoidance of plasmids and expensive inducers for foreign gene expression to reduce production costs; foreign genes are knocked into the chromosome, and their expression is induced with xylose that is present in most biomass feedstock. As loci for the gene knock-in, lacZYA and some pseudogenes are chosen to minimize unexpected effects of… CONTINUE READING

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