Establishment of a novel equine cell line for isolation and propagation of equine herpesviruses.

@article{Maeda2007EstablishmentOA,
  title={Establishment of a novel equine cell line for isolation and propagation of equine herpesviruses.},
  author={Ken Maeda and Shigeru Yasumoto and Akari Tsuruda and Kiyohiko Andoh and Kazushige Kai and Takeshige Otoi and Tomio Matsumura},
  journal={The Journal of veterinary medical science},
  year={2007},
  volume={69 9},
  pages={989-91}
}
In the present study, an equine-derived cell line was established by transfecting primary fetal horse kidney (FHK) cells with expression plasmid encoding simian virus 40 (SV40) large T antigen and then cloning them by limiting dilution. The cloned cell line, named FHK-Tcl3, grew well and could be propagated over 30 times by splitting them 1:3. Equine herpesvirus (EHV)-1 and EHV-4 replicated well in FHK-Tcl3. EHV-2 and EHV-4 were isolated from samples collected from horses in the field using FHK… CONTINUE READING