Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor.

@article{Greene1976EstablishmentOA,
  title={Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor.},
  author={Lloyd A Greene and Arthur S. Tischler},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1976},
  volume={73 7},
  pages={2424-8}
}
A single cell clonal line which responds reversibly to nerve growth factor (NGF) has been established from a transplantable rat adrenal pheochromocytoma. This line, designated PC12, has a homogeneous and near-diploid chromosome number of 40. By 1 week's exposure to NGF, PC12 cells cease to multiply and begin to extend branching varicose processes similar to those produced by sympathetic neurons in primary cell culture. By several weeks of exposure to NGF, the PC12 processes reach 500-1000 mum… CONTINUE READING
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