Establishment of a new method to detect gene expression by laser capture microdissection-assisted single-cell real time RT-PCR without RNA purification

Laser capture microdissection (LCM) combined with real time RT-PCR represents a powerful method for analyzing gene expression levels in selected cell types. To avoid degradation of RNA by endogenous and exogenous RNases and to ensure selection of target cells only, we designed a protocol in which the inactivation of exogenous and endogenous RNases by… CONTINUE READING