Establishment of a gene transfer system for Pseudozyma flocculosa, an antagonistic fungus of powdery mildew fungi

@article{Cheng2001EstablishmentOA,
  title={Establishment of a gene transfer system for Pseudozyma flocculosa, an antagonistic fungus of powdery mildew fungi},
  author={Yanjun Cheng and François Belzile and Philippe Tanguay and Louis Bernier and Richard B{\'e}langer},
  journal={Molecular Genetics and Genomics},
  year={2001},
  volume={266},
  pages={96-102}
}
A reliable DNA-mediated transformation system has been developed for Pseudozyma flocculosa, a fungus that is antagonistic to powdery-mildew fungi. Plasmids harboring various selectable markers under the control of different promoters were tested. Molecular analyses demonstrated that successful transformation could be achieved using a plasmid that confers resistance to hygromycin B under the control of the Ustilago maydis hsp70 promoter and terminator sequences. On average, 1–40 (mean=20… CONTINUE READING
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