Establishment of a chimeric reporting system for the universal detection and high-throughput screening of G protein-coupled receptors.

@article{Wang2009EstablishmentOA,
  title={Establishment of a chimeric reporting system for the universal detection and high-throughput screening of G protein-coupled receptors.},
  author={Chun-Jen Wang and Shih-Han Hsu and Wei-Ting Hung and Ching-Wei Luo},
  journal={Biosensors & bioelectronics},
  year={2009},
  volume={24 7},
  pages={2298-304}
}
G proteins, further divided into four subfamilies (G(s), G(q), G(12) and G(i)) based on their Galpha subunits, are the primary components activated by G protein-coupled receptors (GPCRs). Current GPCR assays are limited to the evaluation of selective Galpha signaling and do not allow comprehensive screening for orphan GPCRs without a known coupled Galpha… CONTINUE READING