Establishment of a cell line and its clonal sublines from a patient with hepatoblastoma.

@article{Doi1976EstablishmentOA,
  title={Establishment of a cell line and its clonal sublines from a patient with hepatoblastoma.},
  author={Ippei Doi},
  journal={Gan},
  year={1976},
  volume={67 1},
  pages={1-10}
}
By cloning from cultivation in vitro of a human hepatoblastoma 3 epithelial cell lines were established, all of which produced alpha-fetoprotein and one of which did albumin in culture. Observation with electron microscopy demonstrated desmosomes and glycogen granules in the cytoplasm of these cells. The morphology, growth characteristics, alpha-fetoprotein production, heterotransplantability, and karyology of the cloned cells suggested that these clonal lines were derived from the tumor cells… CONTINUE READING

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