Establishment of Muc1 Gene Knockout Mouse Model

  title={Establishment of Muc1 Gene Knockout Mouse Model},
  author={BuKai Cheng and Jie Yang and W. -Z. Mao and Jijing Shi and Wei Jin and Xiaodong Liao and Ying Kuang and Ke-Wen Zhao and Lei Huang},
MUCIN1 (MUC1), a member of the mucin family anchored to the apical surface of the epithelial cells, is responsible for immune inflammation and tumorigenesis. A Muc1 knockout mouse model was established for exploring the biological functions and studying mechanisms of MUC1 in tumorigenesis and metastasis. Mouse genomic DNA sequence of the Muc1 gene was obtained using bioinformatics methods. Muc1 gene knockout vector Muc1 -ABRLFn- pBR322 was constructed with exon 2 and exon 3 flanked by two loxP… 
MUC1 triggers lineage plasticity of Her2 positive mammary tumors
It is shown that coexpression of Her2 and MUC1-CD is associated with downregulation of tricarboxylic acid (TCA) cycle genes in tumors, and the reduction in TCA cycle genes induced by MUC 1-CD was found to be significantly connected to poor prognosis in HER2+ breast cancer patients.