Establishment of Leydig cell line, TTE1, from transgenic mice harboring temperature-sensitive simian virus 40 large T-antigen gene.

@article{Ohta2002EstablishmentOL,
  title={Establishment of Leydig cell line, TTE1, from transgenic mice harboring temperature-sensitive simian virus 40 large T-antigen gene.},
  author={Shoichiro Ohta and Yoshiaki Tabuchi and Nobuaki Yanai and Shinji Asano and Hideki Fuse and Masuo Obinata},
  journal={Archives of andrology},
  year={2002},
  volume={48 1},
  pages={43-51}
}
A Leydig cell line, TTE1, has been established from transgenic mice harboring a temperature-sensitive simian virus 40 (tsSV40) large T-antigen gene. The cells grew at a permissive temperature (33 degrees C), but growth was markedly prevented at a nonpermissive temperature (39 degrees C). T-antigen was expressed in the nuclei at 33 degrees C but disappeared at 39 degrees C, indicating that the cells show a temperature-sensitive growth phenotype reflected by the tsSV40 large T-antigen. TTE1 cells… CONTINUE READING