Corpus ID: 86510079

Establishment of Fluorescent SSR Techni que on Detecting Allelic Frequency in Maize (Zea mays L.) Populations with Bulk Sampling Strategy

@inproceedings{Xiaoxin2008EstablishmentOF,
  title={Establishment of Fluorescent SSR Techni que on Detecting Allelic Frequency in Maize (Zea mays L.) Populations with Bulk Sampling Strategy},
  author={Liu Xiao-xin and Xie Chuan-xiao and Zhao Qi and L. Ming and Qu Yan-ying and Z. Bin and Liang Ye-hong and Zhang Shi-huang},
  year={2008}
}
Objective) The objective was to establish the detecting method on allele frequencies in bulk samples. (Method) The abundance of the PCR products in bulk samples were used to represent the frequencies by using fluorescence labeled SSR primers and based on ABI automatic DNA analyzer. (Result) Compared with the conventional SSR technique based on polyacrylamide gel electrophoresis (PAGE) and silver staining, fluorescent SSR was much more sensitive. A total of 82 alleles, and 4.1 alleles per locus… Expand
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