Establishment of Callus and Suspension Culture in Jatropha Curcas Rashida Soomro

  title={Establishment of Callus and Suspension Culture in Jatropha Curcas Rashida Soomro},
  author={RABIA ASMA MEMON},
  • Published 2008
Callus cultures were initiated from leaf and hypocotyl explants isolated from 4 days old seedling of Jatropha curcas L., on Murashige & Skoog (1962) basal medium supplemented with different growth regulator formulations including 2,4-D, BA, GA3, and coconut milk. Excellent growth of callus was obtained in medium supplemented with 0.5mg/L 2, 4-D alone and with 2% v/v coconut milk in hypocotyl explants, Callus produced from hypocotyl explants grew faster during 7 to 30 days of culture then… CONTINUE READING
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