Establishment of Asian citrus psyllid (Diaphorina citri) primary cultures

  title={Establishment of Asian citrus psyllid (Diaphorina citri) primary cultures},
  author={Mizuri Marutani-Hert and Wayne Brian Hunter and David G. Hall},
  journal={In Vitro Cellular \& Developmental Biology - Animal},
The Asian citrus psyllid (AsCP), Diaphorina citri Kuwayama (Hemiptera: Psyllidae), is a highly competent vector of the phloem-inhabiting bacterium Candidatus Liberibacter asiaticus associated with the citrus disease huanglongbing (HLB). Commonly referred to as citrus greening disease in the USA, HLB causes reduced fruit yields, quality, and ultimately tree death and is considered the most serious citrus disease. HLB has become a major limiting factor to the production of citrus worldwide… 

Associated Bacteria of Asian Citrus Psyllid (Hemiptera: Psyllidae: Diaphorina citri)

The microbiome of the Asian citrus psyllid was investigated and four bacteria species detected in cell cultures identified two new types not previously reported to be associated with psyllids, providing new insights into microbial interactions and avenues for developing novel management approaches against the pathogenic bacteria associated with Huanglongbing.

6.11 Development of a Potato Psyllid (Bactericera cockerelli) cell culture

Zebra Complex (ZC), was first documented in potato fields around Saltillo, Mexico in 1994, and first identified in the USA in 2000 in commercial potato fields in Pearsall, TX, where it caused millions of dollars in losses to both potato producers and processors in numerous locations.

Bradyrhizobium isolated from huanglongbing (HLB) affected citrus trees reacts positively with primers for Candidatus Liberibacter asiaticus

In attempts to isolate Las on a low-nutrient medium that had been used for the isolation of several uncultured bacteria of the alpha subclass of proteobacteria, slow-growing Bradyrhizobium spp.

Improved annotation of the insect vector of citrus greening disease: biocuration by a diverse genomics community

To identify and improve gene models as potential targets for pest control, a community effort manually curated several gene families with a focus on genes that have key functional roles in D. citri biology and CLas interactions.

Survey and detection of 'Candidatus Liberibacter asiaticus' in a citrus nursery facility in South Texas.

The results represent the first report of H LB in a nursery facility in Texas, and underscore the need for more intensive surveillance for HLB in citrus nursery stock as an integral component of HLB mitigation efforts in Texas.

Survey of Endosymbionts in the Diaphorina citri Metagenome and Assembly of a Wolbachia wDi Draft Genome

Genome alignment with the complete sequences reveals membership of Wolbachia wDi in supergroup B, further supported by phylogenetic analysis of FtsZ and Wsp phylogenies, supporting the hypothesis that the D. citri introduced into Florida did not originate from China.

Biocuration as an undergraduate training experience: Improving the annotation of the insect vector of Citrus greening disease

To identify and improve gene models as potential targets for pest control, a community effort manually curated several gene families with a focus on genes that have key functional roles in D. citri biology and CLas interactions.

Leafhopper Viral Pathogens: Ultrastructure of salivary gland infection of Taastrup-like virus in Psammotettix alienus Dahlbom; and a novel Rhabdovirus in Homalodisca vitripennis (Germar) Hemiptera: Cicadellidae

Taastrup virus (TV) is a novel virus with a RNA genome, a Filovirus-like morphology, being tentatively placed within the Mononegavirales, the only reported cases of this newly discovered viral pathogen of leafhoppers.

Molecular and cellular analysis of immunity in the phytoplasma vector Euscelidius variegatus: exploiting immunity to improve biological control strategies

Promising results obtained with E. variegaus, a leafhopper frequently used as a laboratory experimental model of insect vector of phytoplasmas, will help in developing in vitro tools for the study of the interactions between these pathogens and their vectors.

Activation of Immune Genes in Leafhoppers by Phytoplasmas and Symbiotic Bacteria

Investigation of the expression of immune genes in the leafhopper vector of phytoplasmas Euscelidius variegatus revealed a specific activation of Raf in midguts after double infection by Asaia and flavescence dorée phy toplasma, supporting the involvement of this gene in the anti-pathogen activity.




Management of citrus greening disease is difficult and requires an integrated approach including use of clean stock, elimination of inoculum via voluntary and regulatory means, use of pesticides to control psyllid vectors in the citrus crop, and biological control of psyllID vectors in non-crop reservoirs.

Detection by PCR of Candidatus Liberibacter asiaticus, the bacterium causing citrus huanglongbing in vector psyllids: application to the study of vector–pathogen relationships

It was concluded that Las persists in the Asian citrus psyllid vector, but is not transovarially transmitted by the vector.

Development of a continuous whitefly cell line [Homoptera: Aleryrodidae: Bemisia tabaci (Gennadius)] for the study of begomovirus.

This is the first report of a continuous cell line established from a species of whitefly and its inoculation with two begomoviruses, and represents great promise for the development of systems that allow researchers to achieve a better understanding of the complex relationship between begomviruses and their whitefly vectors.

Huanglongbing: a destructive, newly-emerging, century-old disease of citrus [Asia; South Africa; Brazil; Florida]

Control of huanglongbing (HLB), a destructive disease of citrus that represents a major threat to the world citrus industry, is preventive and largely based on inoculum elimination by removal of infected trees and chemical treatments against vectors.

Insect cell culture: Improved media and methods for initiating attached cell lines from the lepidoptera

Cell lines from the pupae of the noctuid moth species Spodoptera frugiperda, Heliothis zea, and Trichoplusia ni were progressively adapted to improved media free of insect hemolymph but containing one or more of the following sera: turkey, chicken, and fetal calf.


A detailed account is given of the history, aetiology, biology, epidemiology, detection, geographical distribution, and control of huanglongbing (HLB), a destructive disease of citrus that represents

Primary culture of leafhopper embryonic tissues

Invertebrate tissue culture methods (Springer lab manual)

  • Invertebrate tissue culture methods (Springer lab manual)
  • 2002