Establishment and physiological analyses of photoautotrophic callus cultures of the fern Platycerium coronarium (Koenig) Desv. under CO2 enrichmen

  title={Establishment and physiological analyses of photoautotrophic callus cultures of the fern Platycerium coronarium (Koenig) Desv. under CO2 enrichmen},
  author={Siew-Hwa Kwa and Yeow Chin Wee and Tit Meng Lim and Prakash P. Kumar},
  journal={Journal of Experimental Botany},
Gametophyte-derived callus cultures of Platycerium coronarium could be maintained under photoautotrophic conditions on Murashige and Skoog medium supplemented with 2 mu M 2,4-dichlorophenoxyacetic acid (2,4-D) and with CO2 enrichment. Progressive reduction of sucrose from the medium resulted in a reduction in growth, but an increase in total chlorophyll content. When subculturing was delayed beyond 2 weeks, callus cells differentiated into gametophytes on the medium with less than or equal to 0… Expand
Morphogenetic plasticity of callus reinitiated from cell suspension cultures of the fern Platycerium coronarium
The dark-green gametophytic callus showed a faster rate of growth and morphogenesis as compared to the pale-green sporophyticCallus, and the availability of cell cultures of the same ploidy level, but with two distinct pathways of development will be useful for comparative studies of developmental plasticity. Expand
Ammonium and nitrate uptake and nitrate reductase activity of photoautotrophic callus cultures of the fernPlatycerium coronarium (Koenig) DESV
The uptake of nitrate and ammonium by callus of Platycerium coronarium from the culture medium was examined and the presence of 1% (vol/vol) CO2 generally resulted in the highest rate of Nitrate uptake. Expand
Ribulose-1,5-bisphosphate carboxylase and phosphoenolpyruvate carboxylase activities of photoautotrophic callus of Platycerium coronarium (Koenig ex O.F. Muell.) Desv. under CO2 enrichment
The in vitro activities of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) and phosphoenolpyruvate carboxylase (PEPC) were measured in cell-free extracts of Platycerium coronarium callusExpand
Protocorms of dendroboum sabin H were proliferated on Murashige and Skoog (MS) medium supplemented with 0%,1,5%,3%,5%,7%,9%,11% and 13% sucrose to determine the callus growth , embryogenesis andExpand
Impact of in vitro CO2 enrichment and sugar deprivation on acclimatory responses of Phalaenopsis plantlets to ex vitro conditions.
The results indicate that plantlets grown under CO 2 enrichment developed completely viable photosynthetic apparatus ready to be efficiently transferred to ex vitro condition that has far-reaching implications in micropropagation of Phalaenopsis. Expand
Effect of varying co2 and light levels on growth of hedyotis and sugarcane shoot cultures
The results suggest that Hedyotis shoots in culture exhibit some form of acclimation to high CO2, so that there is no net gain in productivity by photosynthesis. Expand
In vitro sucrose concentration affects growth and acclimatization of Alocasia amazonica plantlets
A sucrose concentration of 3% in the medium is appeared to be better among studied concentrations for both in vitro growth and ex v itro acclimatization of A. amazonica plantlets. Expand
In Vitro Regeneration Systems of Platycerium
In this review, regeneration systems of Platycerium using in vitro techniques are summarized and compared to those among other fern species, and the influence of initial explants, culture media, plant growth regulators, wounding, etc., on morphogenesis, including apospory and apogamy is presented. Expand
Sucrose enhances phosphoenolpyruvate carboxylase activity of in vitro solanum tuberosum L. under non-limiting nitrogen conditions
It is suggested that sucrose metabolism is involved in the replenishment of the tricarboxylic acid cycle by providing carbon skeletons required to sustain phosphoenolpyruvate utilization during high nitrate assimilation. Expand
Effect of indole-3-butyric acid (IBA) on in vitro root induction in dendrobium orchid ( Dendrobium sabin H.)
It is concluded that IBA treatments at lower concentration proved to be effective in root formation of orchid. Expand