Establishment and chimera analysis of 129/SvEv- and C57BL/6-derived mouse embryonic stem cell lines.

@article{Auerbach2000EstablishmentAC,
  title={Establishment and chimera analysis of 129/SvEv- and C57BL/6-derived mouse embryonic stem cell lines.},
  author={Wojtek Auerbach and Judy H. Dunmore and Victoria Fairchild-Huntress and Qingming Fang and Anna B. Auerbach and Dennis Huszar and Alexandra L. Joyner},
  journal={BioTechniques},
  year={2000},
  volume={29 5},
  pages={
          1024-8, 1030, 1032
        }
}
Hundreds of new mutant mouse lines are being produced annually using gene targeting and gene trap approaches in embryonic stem (ES) cells, and the number is expected to continue to grow as the human and mouse genome projects progress. The availability of robust ES cell lines and a simple technology for making chimeras is more attractive now than ever before. We established several new ES cell lines from 129/SvEv and C57BL/6 mice and tested their ability to contribute to the germline following… CONTINUE READING
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