Establishment and characterization of human gastric carcinoma cell lines.

  title={Establishment and characterization of human gastric carcinoma cell lines.},
  author={J. G. Park and Haiyan Yang and Woo Ho Kim and Jake K Chung and Min-soo Kang and Juwon Lee and Jae Hwan Oh and Ho Sung Park and Keng Suan Yeo and S H Kang and Sang Yong Song and Young Kook Kang and Y. J. Bang and Y H Kim and Jungsuh P. Kim},
  journal={International journal of cancer},
  volume={70 4},
We report 8 newly established gastric-carcinoma cell lines (SNU-216, 484, 520, 601, 620, 638, 668, 719) from Korean patients. Morphologic study was carried out using light and electron microscopes. CEA, alpha FP, and CA 19-9 and TPA in supernatant and in cell lysate were measured by radioimmunoassay. p53 and c-Ki-ras gene mutations were screened and confirmed by sequencing. The cell lines, derived from tumors with moderate differentiation, grew as a diffuse monolayer, and those from tumors with… CONTINUE READING


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