Establishment and characterization of embryogenic cell suspension cultures from immature and mature embryos of barley (Hordeum vulgare L.)

@article{Huang2004EstablishmentAC,
  title={Establishment and characterization of embryogenic cell suspension cultures from immature and mature embryos of barley (Hordeum vulgare L.)},
  author={Chunnong Huang and Huajun Yan and Qinfeng Yan and Muyuan Zhu and Miaobao Yuan and Abing Xu},
  journal={Plant Cell, Tissue and Organ Culture},
  year={2004},
  volume={32},
  pages={19-25}
}
Two finely-dispersed, homogeneous and regenerable cell suspension cultures were established from embryogenic callus derived from immature and mature embryos in barley. The quality and viability of suspension cells obtained were determined using differential-interference-contrast microscope and fluorescence microscope. Cell suspension cultures, maintained in modified liquid CC medium, showed a 10-fold increase in dry weight after two weeks with a doubling time of about 3 days. Addition of l… CONTINUE READING
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