Establishment and characterization of photoautotrophic protoplast-derived cultures ofNicotiana plumbaginifolia

Abstract

A procedure is described for the rapid establishment of photoautotrophic protoplast-derived cultures ofNicotiana plumbaginifolia. Photoautotrophic growth was induced by lowering the glucose concentration to 2.5 g.l−1 in the protoplast culture medium and by omitting glucose from the subsequent dilution medium. Four week-old highly viable suspensions were… (More)
DOI: 10.1007/BF00778541

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