Establishment and characterization of an antihuman thyrotropin (TSH) receptor-specific CD4+ T cell line from a patient with Graves' disease: evidence for multiple T cell epitopes on the TSH receptor including the transmembrane domain.

@article{Akamizu1995EstablishmentAC,
  title={Establishment and characterization of an antihuman thyrotropin (TSH) receptor-specific CD4+ T cell line from a patient with Graves' disease: evidence for multiple T cell epitopes on the TSH receptor including the transmembrane domain.},
  author={Takashi Akamizu and Yoshio Ueda and L A Hua and Jun Okuda and Toru Mori},
  journal={Thyroid : official journal of the American Thyroid Association},
  year={1995},
  volume={5 4},
  pages={259-64}
}
From the peripheral lymphocytes of a patient with Graves' disease, we established a T cell line using its reaction to a pool of 49 synthetic peptides corresponding to the entire human thyrotropin receptor (TSHR) sequence. This T cell line showed a specific response to the pool of peptides in a microproliferation assay (stimulation index: 4.8). Flow… CONTINUE READING