Establishment and characterization of a novel method for evaluating gluconeogenesis using hepatic cell lines, H4IIE and HepG2.

Abstract

The liver gluconeogenic pathway is recognized as a target for treating diabetes mellitus. In this study, we attempted to establish a new method to evaluate gluconeogenesis using rat H4IIE hepatoma cells. High-density preculture and exposure to hypertonic solutions, which are known to upregulate the expression of gluconeogenic genes, enhanced glucose release… (More)
DOI: 10.1016/j.abb.2009.09.015

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Cite this paper

@article{Okamoto2009EstablishmentAC, title={Establishment and characterization of a novel method for evaluating gluconeogenesis using hepatic cell lines, H4IIE and HepG2.}, author={Takashi Okamoto and Naohide Kanemoto and Takashi Ban and Toshiki Sudo and Keisuke Nagano and Ichiro Niki}, journal={Archives of biochemistry and biophysics}, year={2009}, volume={491 1-2}, pages={46-52} }