Establishment and characterization of a fibroblast line from Simmental cattle.

@article{Li2009EstablishmentAC,
  title={Establishment and characterization of a fibroblast line from Simmental cattle.},
  author={Lin-Feng Li and Hua Yue and Jianzhang Ma and Wei-jun Guan and Yue-hui Ma},
  journal={Cryobiology},
  year={2009},
  volume={59 1},
  pages={63-8}
}
A fibroblast line (named SCF36) from ear marginal tissue of Simmental cattle was established successfully by direct culture of explants and cell cryopreservation techniques. Biological analysis showed that the population doubling time of the thawed cells was 42.8h. The average viability of the cells was 96.8% before freezing and 91.5% after thawing. Measurements of lactic dehydrogenase and malic dehydrogenase isoenzymes showed no cross-contamination of this cell line with other species… CONTINUE READING

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