Establishment and characterization of a Chinese hamster ovary cell line, CHO-4J, stably expressing a number of Na+/I- symporters.

@article{Kosugi1996EstablishmentAC,
  title={Establishment and characterization of a Chinese hamster ovary cell line, CHO-4J, stably expressing a number of Na+/I- symporters.},
  author={Shinji Kosugi and Narie Sasaki and N. T. Bik Hai and Hideo Sugawa and Naohito Aoki and Chiaki Shigemasa and Tadashi Mori and Akio Yoshida},
  journal={Biochemical and biophysical research communications},
  year={1996},
  volume={227 1},
  pages={94-101}
}
The cDNA of the Na+/I- symporter playing a key role in thyroid iodide transport was cloned very recently. To characterize its function, we transfected the Na+/I- symporter gene into Chinese hamster ovary (CHO) cells and established a cell line stably expressing a number of rat Na+/I- symporters, named CHO-4J. Iodide uptake was dependent on [Na+] and reached a plateau within 30 min. Kinetic studies revealed that the K(m) for iodide was 35 microM, similar to that of FRTL-5 thyroid cells. The… CONTINUE READING
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