Establishment and characterization of 7 ovarian carcinoma cell lines and one granulosa tumor cell line: Growth features and cytogenetics

@article{vandenBergBakker1993EstablishmentAC,
  title={Establishment and characterization of 7 ovarian carcinoma cell lines and one granulosa tumor cell line: Growth features and cytogenetics},
  author={C A van den Berg-Bakker and Anne M. Hagemeijer and Elsa M. Franken‐Postma and Vincent T. H. B. M. Smit and Peter J. K. Kuppen and Hedda van Raavenswaay Claasen and Cees J. Cornelisse and Peter I. Schrier},
  journal={International Journal of Cancer},
  year={1993},
  volume={53}
}
The characteristics of 7 newly established ovarian carcinoma cell lines and one granulosa tumor cell line obtained from tumor samples of 7 patients with varying histology of the primary tumor are reported. The cell lines were isolated from 5 serous carcinomas, a mucinous carcinoma, an endometrioid carcinoma and a granulosa cell tumor. All cell lines were passaged at least 25 times and showed stable growth rates. Colony‐forming efficiency varied on plastic from 2 to 57% and in agar from 0.01 to… 
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The histologic and immunocytochemical comparison of the primary and the relapsed tumor proved evidence of an in vivo change of differentiation from predominantly papillary cystadenocarcinoma to carcinosarcoma.
Establishment and characterization of a human ovarian sarcomatoid carcinoma cell line BUPH:OVSC.
  • H.-F. GuoJ. Feng T. Fu
  • Biology, Medicine
    International journal of gynecological cancer : official journal of the International Gynecological Cancer Society
  • 2005
TLDR
It is shown that the BUPH:OVSC cell line displays unique cellular properties, which make it a useful model for the study of human ovarian sarcomatoid carcinomas.
Establishment and characterization of a human ovarian sarcomatoid carcinoma cell line BUPH: OVSC
  • H. GuoJ. Feng T. Fu
  • Biology, Medicine
    International Journal of Gynecologic Cancer
  • 2004
TLDR
It is shown that the BUPH:OVSC cell line displays unique cellular properties, which make it a useful model for the study of human ovarian sarcomatoid carcinomas.
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Investigating 2D migration, cell cycle parameters and 3D invasion behaviour into different substrates and finding significant differences between the behaviours of cell lines from different origins provides the basis of an in vitro model for identifying differences between ovarian cancer tumour types.
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KGN cells cultured in vitro acquire an aggressive phenotype, which was confirmed by the analysis of cellular activities and the expression of biomarkers, which supported the notion that with passage, KGN cells became more aggressive.
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The culture approach outlined herein will facilitate generation of reagents useful for many aspects of ovarian cancer biology, particularly with respect to growth properties, antigen expression, and genomic alterations.
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Comprehensive profiling of 39 ovarian cancer cell lines under controlled, uniform conditions demonstrates clinically relevant cellular and genomic characteristics, providing a rational basis for selecting models to develop specific treatment approaches for histological and molecular subtypes of ovarian cancer.
Nuclear Receptor Profiling of Ovarian Granulosa Cell Tumors
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A full profile of NR expression in GCT will enable full characterization of their roles and potential as therapeutic targets and the concordance of expression for each NR across the tumors is remarkably high with same discordance between the cell lines and the tumors.
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