Establishment and application of a novel T cell clonality analysis using single-strand conformation polymorphism of T cell receptor messenger signals.

@article{Yamamoto1996EstablishmentAA,
  title={Establishment and application of a novel T cell clonality analysis using single-strand conformation polymorphism of T cell receptor messenger signals.},
  author={Keisuke Yamamoto and Kayo Masuko-hongo and Atsushi Tanaka and Manae Kurokawa and Torsten Alfons H{\"o}ger and Kusuki Nishioka and Tomohiro Kato},
  journal={Human immunology},
  year={1996},
  volume={48 1-2},
  pages={23-31}
}
To identify the existence of antigen specific T cell responses and to follow the changes of these reactions, it is considered useful to evaluate whether certain T cells clonally accumulate in the lymphocyte population. For this purpose, we have established a novel method to analyze T cell clonality using a combination of reverse transcriptase-polymerase chain reaction of T cell receptor beta chain transcripts and single-strand conformation polymorphism (SSCP). Using this method, we obtained a… CONTINUE READING
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