Corpus ID: 88056442

Establishment and Application of a Double RT-PCR for ARV and ALV

@inproceedings{Chaoying2013EstablishmentAA,
  title={Establishment and Application of a Double RT-PCR for ARV and ALV},
  author={Ma Chao-ying},
  year={2013}
}
To identify and differentiate rapidly the cause of clinical diseases,a double RT-PCR was developed.Two sets of specific primers were designed according to the sequences of avian reovirus(ARV) and avian leukosis virus(ALV) in the GenBank.A double polymerase chain reaction was optimized to simultaneously detect two pathogens of ARV and ALV in this article.The double RT-PCR system would amplify a 485bp fragment for ARV and a 673bp for ALV simultaneously or separately in the samples,depending on… Expand