Establishing a new methodology for genome mining and biosynthesis of polyketides and peptides through yeast molecular genetics.

@article{Ishiuchi2012EstablishingAN,
  title={Establishing a new methodology for genome mining and biosynthesis of polyketides and peptides through yeast molecular genetics.},
  author={Kan'ichiro Ishiuchi and Takehito Nakazawa and Takashi Ookuma and Satoru Sugimoto and Michio Sato and Yuta Tsunematsu and Noriyasu Ishikawa and Hiroshi Noguchi and Kinya Hotta and Hisao Moriya and Kenji Watanabe},
  journal={Chembiochem : a European journal of chemical biology},
  year={2012},
  volume={13 6},
  pages={846-54}
}
Fungal genome sequencing has revealed many genes coding for biosynthetic enzymes, including polyketide synthases and nonribosomal peptide synthetases. However, characterizing these enzymes and identifying the compounds they synthesize remains a challenge, whether the genes are expressed in their original hosts or in more tractable heterologous hosts, such as yeast. Here, we developed a streamlined method for isolating biosynthetic genes from fungal sources and producing bioactive molecules in… CONTINUE READING

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