Establishing a TaqMan-Based Real-Time PCR Assay for the rapid detection and quantification of the newly emerged duck tembusu virus

@inproceedings{Yan2011EstablishingAT,
  title={Establishing a TaqMan-Based Real-Time PCR Assay for the rapid detection and quantification of the newly emerged duck tembusu virus},
  author={Liping Yan and Pixi Yan and Jiewen Zhou and Qiaoyang Teng and Zejun Li},
  booktitle={Virology Journal},
  year={2011}
}
To establish an accurate, rapid, and a quantifiable method for the detection of the newly emerged duck Tembusu virus (DTMUV) that recently caused a widespread infectious disease in ducks in China, we developed a TaqMan-based real-time PCR assay by using E gene-specific primers and a TaqMan probe. This real-time PCR assay was 100 times more sensitive than… CONTINUE READING