Established method of chondroitin sulphate extraction from buffalo (Bubalus bubalis) cartilages and its identification by FTIR

@inproceedings{Sundaresan2018EstablishedMO,
  title={Established method of chondroitin sulphate extraction from buffalo (Bubalus bubalis) cartilages and its identification by FTIR},
  author={G. Sundaresan and Robinson J. J. Abraham and V. Appa Rao and R. Narendra Babu and Varun Govind and Mahantesh F. Meti},
  booktitle={Journal of food science and technology},
  year={2018}
}
A study was conducted for extraction of chondroitin sulphate (CS) from buffalo tracheal, nasal and joint cartilages. CS was extracted from cartilages using 0.25% papain digestion, dialyzed, precipitated with 10% TCA and finally lyophilized to dry powder. Dimethylmethylene blue assay was performed to estimate the quantity of CS extracted. Identification of extracted CS was performed with SDS-PAGE and Fourier transforms infrared spectroscopy (FTIR). SDS-PAGE analysis of extracted CS revealed… CONTINUE READING
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