Essentiality of ribosomal and transcription antitermination proteins analyzed by systematic gene replacement in Escherichia coli.


We describe here details of the method we used to identify and distinguish essential from nonessential genes on the bacterial Escherichia coli chromosome. Three key features characterize our method: high-efficiency recombination, precise replacement of just the open reading frame of a chromosomal gene, and the presence of naturally occurring duplications… (More)


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