Essential role of Tip60-dependent recruitment of ribonucleotide reductase at DNA damage sites in DNA repair during G1 phase.

@article{Niida2010EssentialRO,
  title={Essential role of Tip60-dependent recruitment of ribonucleotide reductase at DNA damage sites in DNA repair during G1 phase.},
  author={Hiroyuki Niida and Yuko Katsuno and Misuzu Sengoku and Midori Shimada and Megumi Yukawa and Masae Ikura and Tsuyoshi Ikura and Kazuteru Kohno and Hiroki Shima and Hidekazu Suzuki and Satoshi Tashiro and Makoto Nakanishi},
  journal={Genes & development},
  year={2010},
  volume={24 4},
  pages={333-8}
}
A balanced deoxyribonucleotide (dNTP) supply is essential for DNA repair. Here, we found that ribonucleotide reductase (RNR) subunits RRM1 and RRM2 accumulated very rapidly at damage sites. RRM1 bound physically to Tip60. Chromatin immunoprecipitation analyses of cells with an I-SceI cassette revealed that RRM1 bound to a damage site in a Tip60-dependent manner. Active RRM1 mutants lacking Tip60 binding failed to rescue an impaired DNA repair in RRM1-depleted G1-phase cells. Inhibition of RNR… CONTINUE READING