Essential role of His163 of cytochrome P450 1A2 in catalytic functions associated with cytochrome b5.

The effect of cytochrome b5 on the activity of cytochrome P450 1A2 (P450 1A2) and three site-directed mutants designed to alter surface charges has been investigated. Cytochrome b5 increased the rate of oxidation of methanol and/or 7-ethoxycoumarin 2- to 10-fold in both wild type and mutant P450. Cytochrome b5 also increased the coupling of electron… CONTINUE READING