Essential role for de novo DNA methyltransferase Dnmt3a in paternal and maternal imprinting

@article{Kaneda2004EssentialRF,
  title={Essential role for de novo DNA methyltransferase Dnmt3a in paternal and maternal imprinting},
  author={Masahiro Kaneda and Masaki Okano and Kenichiro Hata and Takashi Sado and Naomi Tsujimoto and En Li and Hiroyuki Sasaki},
  journal={Nature},
  year={2004},
  volume={429},
  pages={900-903}
}
Imprinted genes are epigenetically marked during gametogenesis so that they are exclusively expressed from either the paternal or the maternal allele in offspring. Imprinting prevents parthenogenesis in mammals and is often disrupted in congenital malformation syndromes, tumours and cloned animals. Although de novo DNA methyltransferases of the Dnmt3 family are implicated in maternal imprinting, the lethality of Dnmt3a and Dnmt3b knockout mice has precluded further studies. We here report the… 

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TLDR
A set of unique de novo DNA methylation target sites for both DNMT3 enzymes during mammalian development that overlap with hypomethylated sites in human patients are reported.
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TLDR
This thesis unambiguously shows that genomic imprints are essential for placental development and engineered a novel targeted deletion of the imprinted Klf14 gene and found it has an effect on placental growth.
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