Essential large transcripts of the maize Spm transposable element are generated by alternative splicing.

@article{Masson1989EssentialLT,
  title={Essential large transcripts of the maize Spm transposable element are generated by alternative splicing.},
  author={Ph. Masson and George Rutherford and Jo Ann Banks and Nina Fedoroff},
  journal={Cell},
  year={1989},
  volume={58 4},
  pages={755-65}
}
We used in vitro mutagenesis and cDNA cloning to identify new Suppressor-mutator (Spm) transposable element genes. Frameshift mutations in the ORFs of the tnpA gene's first intron markedly reduce Spm activity in transgenic tobacco, indicating that intron sequences encode essential gene products. Evidence is given that Spm encodes large alternatively spliced transcripts, designated tnpB (4.9 kb), tnpC (5.7 kb), and tnpD (5.8 kb), comprising all of the tnpA exons, most of the tnpA intron 1 ORF1… CONTINUE READING

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