Essential alterations of heparan sulfate during the differentiation of embryonic stem cells to Sox1-enhanced green fluorescent protein-expressing neural progenitor cells.

@article{Johnson2007EssentialAO,
  title={Essential alterations of heparan sulfate during the differentiation of embryonic stem cells to Sox1-enhanced green fluorescent protein-expressing neural progenitor cells.},
  author={Claire E Johnson and Brett E. Crawford and Marios P. Stavridis and Gerdy B. ten Dam and Annie L Wat and Graham Rushton and Christopher M Ward and Valerie Wilson and Toin H van Kuppevelt and Jeffrey D Esko and Austin Smith and John Thomas Gallagher and Catherine L. R. Merry},
  journal={Stem cells},
  year={2007},
  volume={25 8},
  pages={1913-23}
}
Embryonic stem (ES) cells can be cultured in conditions that either maintain pluripotency or allow differentiation to the three embryonic germ layers. Heparan sulfate (HS), a highly polymorphic glycosaminoglycan, is a critical cell surface coreceptor in embryogenesis, and in this paper we describe its structural transition from an unusually low-sulfated variant in ES cells to a more highly sulfated form in fluorescence-activated cell sorting-purified neural progenitor cells. The characteristic… CONTINUE READING