Escherichia coli single-strand binding protein forms multiple, distinct complexes with single-stranded DNA.

@article{Bujalowski1986EscherichiaCS,
  title={Escherichia coli single-strand binding protein forms multiple, distinct complexes with single-stranded DNA.},
  author={Wlodzimierz Bujalowski and Timothy M. Lohman},
  journal={Biochemistry},
  year={1986},
  volume={25 24},
  pages={7799-802}
}
Four distinct binding modes for the interaction of Escherichia coli single-strand binding (SSB) protein with single-stranded (ss) DNA have been identified on the basis of quantitative titrations that monitor the quenching of the SSB protein fluorescence upon binding to the homopolynucleotide poly(dT) over a range of MgCl2 and NaCl concentrations at 25 and 37 degrees C. This is the first observation of multiple binding modes for a single protein binding to DNA. These results extend previous… CONTINUE READING
Highly Influential
This paper has highly influenced a number of papers. REVIEW HIGHLY INFLUENTIAL CITATIONS

2 Figures & Tables

Connections & Topics

Mentioned Connections BETA
Four distinct binding modes for the interaction of Escherichia coli single - strand binding ( SSB ) protein with single - stranded ( ss ) DNA have been identified on the basis of quantitative titrations that monitor the quenching of the SSB protein fluorescence upon binding to the homopolynucleotide poly(dT ) over a range of MgCl2 and NaCl concentrations at 25 and 37 degrees C. This is the first observation of multiple binding modes for a single protein binding to DNA .
Four distinct binding modes for the interaction of Escherichia coli single - strand binding ( SSB ) protein with single - stranded ( ss ) DNA have been identified on the basis of quantitative titrations that monitor the quenching of the SSB protein fluorescence upon binding to the homopolynucleotide poly(dT ) over a range of MgCl2 and NaCl concentrations at 25 and 37 degrees C. This is the first observation of multiple binding modes for a single protein binding to DNA .
All Topics