Escherichia coli isolates from inflammatory bowel diseases patients survive in macrophages and activate NLRP3 inflammasome.

@article{Fuente2014EscherichiaCI,
  title={Escherichia coli isolates from inflammatory bowel diseases patients survive in macrophages and activate NLRP3 inflammasome.},
  author={M. De la Fuente and L. Franchi and D. Araya and David D{\'i}az-Jim{\'e}nez and M. Olivares and M. {\'A}lvarez-Lobos and D. Golenbock and Mar{\'i}a-Julieta Gonz{\'a}lez and F. L{\'o}pez-Kostner and R. Quera and G. N{\'u}{\~n}ez and R. Vidal and M. Hermoso},
  journal={International journal of medical microbiology : IJMM},
  year={2014},
  volume={304 3-4},
  pages={
          384-92
        }
}
Crohn's disease (CD) is a multifactorial pathology associated with the presence of adherent-invasive Escherichia coli (AIEC) and NLRP3 polymorphic variants. The presence of intracellular E. coli in other intestinal pathologies (OIP) and the role of NLRP3-inflammasome in the immune response activated by these bacteria have not been investigated. In this study, we sought to characterize intracellular strains isolated from patients with CD, ulcerative colitis (UC) and OIP, and analyze NLRP3… Expand

Paper Mentions

Observational Clinical Trial
Background: There are about 100 trillion microbial cells in a person s gut. This is called the human gut microbiota. When this is disrupted, it can lead to many diseases… Expand
ConditionsHepatocellular Carcinoma, Liver Cancer, Liver Cell Caricinoma, (+1 more)
Adherent-invasive Escherichia coli (AIEC) in pediatric Crohn’s disease patients: phenotypic and genetic pathogenic features
TLDR
The finding that AIEC-like isolates were more abundant in CD patients, indicates that a close association of these strains with CD may also exists in pediatric patients and confirmed the “pathobiont” nature of AIEC strains. Expand
Virulence determinants and genetic diversity of adherent-invasive Escherichia coli (AIEC) strains isolated from patients with Crohn's disease.
TLDR
It was found that chuA and ratA genes were the most significant genetic markers associated with AIEC compared to non-AIEC strains isolated from CD patients and HC p = 0.0119, 0.0094 respectively. Expand
Adherent-invasive Escherichia coli in inflammatory bowel disease
Intestinal microbiome dysbiosis has been consistently described in patients with IBD. In the last decades, Escherichia coli, and the adherent-invasive E coli (AIEC) pathotype in particular, has beenExpand
Glucocorticoids Impair Phagocytosis and Inflammatory Response Against Crohn’s Disease-Associated Adherent-Invasive Escherichia coli
TLDR
It is demonstrated that glucocorticoids impair phagocytosis and induce anti-inflammatory polarization after AIEC infection, possibly contributing to the survival of AIEC in infected CD patients. Expand
Comparative genomics of a subset of Adherent/Invasive Escherichia coli strains isolated from individuals without inflammatory bowel disease.
TLDR
It is plausible to suggest that non-IBD AIEC strains belong to a different pathotype, based on the differences in virulence determinants and SNPs, which indicates the emergence of these strains apart from the AIEC group. Expand
Macrophages Inability to Mediate Adherent-Invasive E. coli Replication is Linked to Autophagy in Crohn’s Disease Patients
TLDR
The macrophage defect to mediate AIEC clearance in CD patients is linked to polymorphisms related to autophagy such as IRGM and ULK-1, which led to an increase of pro-inflammatory cytokines IL-1β and TNF-α in CD macrophages. Expand
Monocyte-derived macrophages from Crohn's disease patients are impaired in the ability to control intracellular adherent-invasive Escherichia coli and exhibit disordered cytokine secretion profile.
TLDR
MDM from CD patients are unable to restrict intracellular AIEC replication, leading to disordered inflammatory response influenced by disease activity, including IL-6 and TNF-alpha secretion. Expand
The IbeA Invasin of Adherent-Invasive Escherichia coli Mediates Interaction with Intestinal Epithelia and Macrophages
TLDR
The identified ibeA gene in the genome of AIEC strain NRG857c is identified and the contribution of IbeA to the interaction ofAIEC with IECs and macrophages and colonization of the mouse intestine is reported, supporting the function of I beA in the AIEC invasion process, macrophage survival, and inflammatory response in the murine intestine. Expand
Role of Inflammasomes in Intestinal Inflammation and Crohn's Disease
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Evidence linking NLRP3, NLRC4, and NLRP6 inflammasomes to intestinal inflammation is reviewed to provide a basis for new hypotheses to explain how genetic polymorphism associated with Crohn's disease modulate intestinal inflammation. Expand
Effects of a pathogenic ETEC strain and a probiotic Enterococcus faecium strain on the inflammasome response in porcine dendritic cells.
TLDR
It is concluded that ETEC, but not E. faecium, was able to stimulate inflammasome components in porcine monocyte-derived DC, and preincubation with the probiotic did not influence NLRP3 inflammaome activation in comparison with cells infected with ETEC alone. Expand
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