Escherichia coli-induced alterations of human spermatozoa. An electron microscopy analysis.

@article{Diemer2000EscherichiaCA,
  title={Escherichia coli-induced alterations of human spermatozoa. An electron microscopy analysis.},
  author={Thorsten Diemer and P. Huwe and Hans Wilhelm Michelmann and Frank Mayer and Hans Gerd Schiefer and Wolfgang Weidner},
  journal={International journal of andrology},
  year={2000},
  volume={23 3},
  pages={178-86}
}
This study evaluated if the negative influence of Escherichia coli on the motility of human spermatozoa is a consequence of E. coli-induced ultrastructural alterations. Suspensions of spermatozoa were artificially infected with E. coli from a serotyped, pathogenic strain and incubated at 37 degrees C for 6 h. After incubation, spermatozoa were fixed in glutaraldehyde, stained with osmium tetroxide and ruthenium red and embedded in Spurr(R)-resin followed by ultramicrotomy. The sections were… CONTINUE READING