Escherichia coli Vertebral Osteomyelitis Diagnosed According to Broad-range 16S rRNA Gene Polymerase Chain Reaction (PCR).

Abstract

Identifying the causative agent of pyogenic osteomyelitis is often challenging, especially when antibiotics are administered before a biopsy. We herein present a case of osteomyelitis in the cervical vertebrae presenting with progressive paralytic symptoms, in which we successfully identified Escherichia coli from a biopsy specimen using broad-range 16S rRNA gene polymerase chain reaction (PCR) even though sensitive antibiotics had been used for more than 50 days before the biopsy. Broad-range 16S rRNA gene PCR is a useful diagnostic method, especially when prebiopsy antibiotics are unavoidably used for a clinically unstable state.

DOI: 10.2169/internalmedicine.54.5066

Cite this paper

@article{Shibata2015EscherichiaCV, title={Escherichia coli Vertebral Osteomyelitis Diagnosed According to Broad-range 16S rRNA Gene Polymerase Chain Reaction (PCR).}, author={Satoshi Shibata and Ryutaro Tanizaki and Koji Watanabe and Kenta Makabe and Naoki Shoda and Satoshi Kutsuna and M. Nagamatsu and Shinichi Oka and Norio Ohmagari}, journal={Internal medicine}, year={2015}, volume={54 24}, pages={3237-40} }