Escherichia coli SeqA protein affects DNA topology and inhibits open complex formation at oriC.

@article{Torheim1999EscherichiaCS,
  title={Escherichia coli SeqA protein affects DNA topology and inhibits open complex formation at oriC.},
  author={N K Torheim and Kirsten Skarstad},
  journal={The EMBO journal},
  year={1999},
  volume={18 17},
  pages={
          4882-8
        }
}
Chromosome replication in Escherichia coli is initiated by the DnaA protein. Binding of DnaA to the origin, oriC, followed by formation of an open complex are the first steps in the initiation process. Based on in vivo studies the SeqA protein has been suggested to function negatively in the initiation of replication, possibly by inhibiting open complex formation. In vitro studies have shown that SeqA inhibits oriC-dependent replication. Here we show by KMnO(4) probing that SeqA inhibits open… CONTINUE READING

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