Escherichia coli RecX inhibits RecA recombinase and coprotease activities in vitro and in vivo.

@article{Stohl2003EscherichiaCR,
  title={Escherichia coli RecX inhibits RecA recombinase and coprotease activities in vitro and in vivo.},
  author={Elizabeth A. Stohl and Joel P. Brockman and Kristin L Burkle and Katsumi Morimatsu and Stephen C Kowalczykowski and H Steven Seifert},
  journal={The Journal of biological chemistry},
  year={2003},
  volume={278 4},
  pages={2278-85}
}
In Escherichia coli the RecA protein plays a pivotal role in homologous recombination, DNA repair, and SOS repair and mutagenesis. A gene designated recX (or oraA) is present directly downstream of recA in E. coli; however, the function of RecX is unknown. In this work we demonstrated interaction of RecX and RecA in a yeast two-hybrid assay. In vitro, substoichiometric amounts of RecX strongly inhibited both RecA-mediated DNA strand exchange and RecA ATPase activity. In vivo, we showed that… CONTINUE READING

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