Escherichia coli RNA polymerase core and holoenzyme structures.

@article{Finn2000EscherichiaCR,
  title={Escherichia coli RNA polymerase core and holoenzyme structures.},
  author={Robert D. Finn and Elena V Orlova and Brent E. Gowen and Martin Buck and Marin van Heel},
  journal={The EMBO journal},
  year={2000},
  volume={19 24},
  pages={6833-44}
}
Multisubunit RNA polymerase is an essential enzyme for regulated gene expression. Here we report two Escherichia coli RNA polymerase structures: an 11.0 A structure of the core RNA polymerase and a 9.5 A structure of the sigma(70) holoenzyme. Both structures were obtained by cryo-electron microscopy and angular reconstitution. Core RNA polymerase exists in an open conformation. Extensive conformational changes occur between the core and the holoenzyme forms of the RNA polymerase, which are… CONTINUE READING

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