Escherichia coli NifS-like proteins provide selenium in the pathway for the biosynthesis of selenophosphate.

@article{Lacourciere2000EscherichiaCN,
  title={Escherichia coli NifS-like proteins provide selenium in the pathway for the biosynthesis of selenophosphate.},
  author={Gerard M Lacourciere and Hisaaki Mihara and Tatsuo Kurihara and Nobuyoshi Esaki and Thressa Campbell Stadtman},
  journal={The Journal of biological chemistry},
  year={2000},
  volume={275 31},
  pages={23769-73}
}
Selenophosphate synthetase (SPS), the selD gene product from Escherichia coli, catalyzes the biosynthesis of monoselenophosphate, AMP, and orthophosphate in a 1:1:1 ratio from selenide and ATP. Kinetic characterization revealed the K(m) value for selenide approached levels that are toxic to the cell. Our previous demonstration that a Se(0)-generating system consisting of l-selenocysteine and the Azotobacter vinelandii NifS protein can replace selenide for selenophosphate biosynthesis in vitro… CONTINUE READING

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