Escherichia coli F4 fimbriae specific llama single-domain antibody fragments effectively inhibit bacterial adhesion in vitro but poorly protect against diarrhoea.

@article{Harmsen2005EscherichiaCF,
  title={Escherichia coli F4 fimbriae specific llama single-domain antibody fragments effectively inhibit bacterial adhesion in vitro but poorly protect against diarrhoea.},
  author={Michiel M. Harmsen and Conny B. van Solt and Ayla Hoogendoorn and Fred G. van Zijderveld and Theo A. Niewold and Jan van der Meulen},
  journal={Veterinary microbiology},
  year={2005},
  volume={111 1-2},
  pages={89-98}
}
Oral administration of polyclonal antibodies directed against enterotoxigenic Escherichia coli (ETEC) F4 fimbriae is used to protect against piglet post-weaning diarrhoea. For cost reasons, we aim to replace these polyclonal antibodies by recombinant llama single-domain antibody fragments (VHHs) that can be produced efficiently in microorganisms. Six F4 fimbriae specific VHHs were isolated. The VHH that was produced at the highest level by yeast, K609, was further analysed. 3.8 mg/L K609… CONTINUE READING
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