Errors in the bisulfite conversion of DNA: modulating inappropriate- and failed-conversion frequencies

@inproceedings{Genereux2008ErrorsIT,
  title={Errors in the bisulfite conversion of DNA: modulating inappropriate- and failed-conversion frequencies},
  author={Diane P Genereux and Winslow C. Johnson and Alice F. Burden and Reinhard St{\"o}ger and Charles Laird},
  booktitle={Nucleic acids research},
  year={2008}
}
Bisulfite treatment can be used to ascertain the methylation states of individual cytosines in DNA. Ideally, bisulfite treatment deaminates unmethylated cytosines to uracils, and leaves 5-methylcytosines unchanged. Two types of bisulfite-conversion error occur: inappropriate conversion of 5-methylcytosine to thymine, and failure to convert unmethylated cytosine to uracil. Conventional bisulfite treatment requires hours of exposure to low-molarity, low-temperature bisulfite ('LowMT') and… CONTINUE READING

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