Equivalence test in quantitative reverse transcription polymerase chain reaction: confirmation of reference genes suitable for normalization.

@article{Haller2004EquivalenceTI,
  title={Equivalence test in quantitative reverse transcription polymerase chain reaction: confirmation of reference genes suitable for normalization.},
  author={Florian Haller and Bettina Kulle and Stefanie Schwager and Bastian Gunawan and Anja von Heydebreck and Holger S{\"u}ltmann and L{\'a}szl{\'o} F{\"u}zesi},
  journal={Analytical biochemistry},
  year={2004},
  volume={335 1},
  pages={1-9}
}
In quantitative reverse transcription-polymerase chain reaction (qRT-PCR), normalization using reference genes is a common useful approach, but the validation of suitable reference genes remains a crucial problem. Use of unconfirmed reference genes may lead to misinterpretation of the expression of target genes. The aim of this study was to adapt an adequate statistical approach to identify and validate reference genes suitable for normalization in qRT-PCR assays. We introduce the equivalence… CONTINUE READING