Equine trypsin: purification and development of a radio-immunoassay.

  title={Equine trypsin: purification and development of a radio-immunoassay.},
  author={Sigrid Grulke and G Deby-dupont and Monika C Gangl and Thierry J Franck and Carol M T Deby and Didier D A Serteyn},
  journal={Veterinary research},
  volume={34 3},
Shock is accompanied by generalised splanchnic hypoperfusion, and splanchnic organs like the pancreas can be damaged, as shown in animal experimental models and in humans, by the presence of high plasma concentrations of trypsin and other pancreatic enzymes. In order to design a radioimmunoassay technique (RIA) for the measurement of equine trypsin-like immunoreactivity (TLI) in biological fluids, trypsin was purified (with purity > or = 96%) from the equine pancreas by extraction in an acid… CONTINUE READING