Equilibrium properties of mouse-Torpedo acetylcholine receptor hybrids expressed in Xenopus oocytes

@article{Yoshii1987EquilibriumPO,
  title={Equilibrium properties of mouse-Torpedo acetylcholine receptor hybrids expressed in Xenopus oocytes},
  author={Kiyonori Yoshii and L Yu and K M Mayne and Norman Davidson and Henry A. Lester},
  journal={The Journal of General Physiology},
  year={1987},
  volume={90},
  pages={553 - 573}
}
This study used messenger RNA encoding each subunit (alpha, beta, gamma and delta) of the nicotinic acetylcholine (ACh) receptor from mouse BC3H-1 cells and from Torpedo electric organ. The mRNA was synthesized in vitro by transcription with SP6 polymerase from cDNA clones. All 16 possible combinations that include one mRNA for each of alpha, beta, gamma, and delta were injected into oocytes. After allowing 2-3 d for translation and assembly, we assayed each oocyte for (a) receptor assembly… CONTINUE READING

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Channel duration mainly determines voltage sensitivity in mouse - Torpedo acetylcholine receptor hybrids

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1987

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Location of a delta - subunit region determining ion - transport through the acetylcholine - receptor channel

J. Mudd, V. Shah
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