Equilibrium properties of mouse-Torpedo acetylcholine receptor hybrids expressed in Xenopus oocytes

  title={Equilibrium properties of mouse-Torpedo acetylcholine receptor hybrids expressed in Xenopus oocytes},
  author={Kiyonori Yoshii and L Yu and K M Mayne and Norman Davidson and Henry A. Lester},
  journal={The Journal of General Physiology},
  pages={553 - 573}
This study used messenger RNA encoding each subunit (alpha, beta, gamma and delta) of the nicotinic acetylcholine (ACh) receptor from mouse BC3H-1 cells and from Torpedo electric organ. The mRNA was synthesized in vitro by transcription with SP6 polymerase from cDNA clones. All 16 possible combinations that include one mRNA for each of alpha, beta, gamma, and delta were injected into oocytes. After allowing 2-3 d for translation and assembly, we assayed each oocyte for (a) receptor assembly… CONTINUE READING

From This Paper

Figures, tables, and topics from this paper.


Publications citing this paper.


Publications referenced by this paper.
Showing 1-10 of 30 references

Channel duration mainly determines voltage sensitivity in mouse - Torpedo acetylcholine receptor hybrids

N. Davidson, H. A. Lester

Isolation and characterization of cDNA clone for the complete coding region of the ~ subunit of the mouse acetylcholine receptor

J. Nargeot, T. P. Snutch, N. Davidson, H. A. Lester
Proceedings of the National Academy of Sciences . • 1987

The nicotinic acetylcholine receptor : gene expression and ion channel function

L. Yu
View 1 Excerpt

Location of a delta - subunit region determining ion - transport through the acetylcholine - receptor channel

J. Mudd, V. Shah

Similar Papers

Loading similar papers…