Equilibrium measurements of the interactions of guanine nucleotides with Escherichia coli elongation factor G and the ribosome.

@article{Baca1976EquilibriumMO,
  title={Equilibrium measurements of the interactions of guanine nucleotides with Escherichia coli elongation factor G and the ribosome.},
  author={Oswald G. Baca and Michael S. Rohrbach and James W. Bodley},
  journal={Biochemistry},
  year={1976},
  volume={15 21},
  pages={
          4570-4
        }
}
The interactions among Escherichia coli elongation factor G (EF-G), guanine nucleotides, ribosomes, and fusidic acid were investigated by a number of physical techniques. Equilibrium dialysis studies demonstrated the existence of a binary EF-G-GDP complex. This complex forms with a stoichiometry of ca 1:1 and an apparent Ka of 2.5 X 10(5) M-1. While no evidence was obtained for the formation of a ribosome-GDP complex, in the presence of ribosomes, the apparent Ka for guanosine diphosphate (GDP… CONTINUE READING

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