Equilibrium binding assays reveal the elevated stoichiometry and salt dependence of the interaction between full-length human sex-determining region on the Y chromosome (SRY) and DNA.

@article{Baud2002EquilibriumBA,
  title={Equilibrium binding assays reveal the elevated stoichiometry and salt dependence of the interaction between full-length human sex-determining region on the Y chromosome (SRY) and DNA.},
  author={St{\'e}phanie Baud and Emmanuel Margeat and Serge Lumbroso and Françoise Paris and Charles S Sultan and Catherine A. Royer and Nicolas Poujol},
  journal={The Journal of biological chemistry},
  year={2002},
  volume={277 21},
  pages={18404-10}
}
In an effort to better define the molecular mechanism of the functional specificity of human sex-determining region on the Y chromosome (SRY), we have carried out equilibrium binding assays to study the interaction of the full-length bacterial-expressed protein with a DNA response element derived from the CD3epsilon gene enhancer. These assays are based on the observation of the fluorescence anisotropy of a fluorescein moiety covalently bound to the target oligonucleotide. The low anisotropy… CONTINUE READING