Epstein-Barr–based episomal chromosomes shuttle 100 kb of self-replicating circular human DNA in mouse cells

@article{Kelleher1998EpsteinBarrbasedEC,
  title={Epstein-Barr–based episomal chromosomes shuttle 100 kb of self-replicating circular human DNA in mouse cells},
  author={Zachary T. Kelleher and Haiyan Fu and Elizabeth Livanos and Brian Wendelburg and Sara Gulino and J H Vos},
  journal={Nature Biotechnology},
  year={1998},
  volume={16},
  pages={762-768}
}
We describe the microcell fusion transfer of 100–200 kb self-replicating circular human minichromosomes from human into mouse cells. This experimental approach is illustrated through the shuttling of the latent 170 kb double-stranded DNA genome from the human herpesvirus, Epstein-Barr virus, into nonpermissive rodent cells. Using this interspecies transfer strategy, circular episomes carrying 95–105 kb of human DNA were successfully established at low copy number in mouse A9 cells. Selected… CONTINUE READING

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This experimental approach is illustrated through the shuttling of the latent 170 kb double - stranded DNA genome from the human herpesvirus , Epstein - Barr virus , into nonpermissive rodent cells .
This experimental approach is illustrated through the shuttling of the latent 170 kb double - stranded DNA genome from the human herpesvirus , Epstein - Barr virus , into nonpermissive rodent cells .
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